| Application | ProteoQwest Colorimetric Western Blotting Kit, BCIP/NBT Substrate is designed for high sensitivity colorimetric detection of as little as 0.25 ng of protein. The colorimetric reaction occurs directly on the PVDF or nitrocellulose membrane; no darkroom or film is needed. All of the components of this ProteoQwest kit have been extensively tested and optimized. This kit is designed for 25 mini-gel sized (10 × 10 cm) blots. It is possible to use this kit for as many as 45 blots if half the suggested amount of reagents is used. |
| Features and Benefits | • Short procedure time
• Reproducible results
• Minimal background
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| Other Notes | Store the reagents as supplied in the kit at 2-8 °C, except for the Chemichrome Western Control which should be stored in the freezer at −20 °C. The unreconstituted packets and reagents are stable for 1 year. Once reconstituted, the Tris buffered saline with Tween® 20 is stable for 2 weeks at 2-8 °C if filtered into a sterile container using a 0.2 μm sterile filter. Once reconstituted, the Tris buffered saline with 3% milk is stable for 1 day at 2-8 °C. |
| Legal Information | BCIP is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
| | TWEEN is a registered trademark of Croda International PLC |
| | ProteoQwest is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
